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Ultrasonic-augmented Primary Adult Fibroblast Isolation Video (Video) | JoVE

DOI :

10.3791/59858-v

July 29th, 2019

July 29th, 2019

6,926 Views

1Institute of Pharmacology and Toxicology, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden

We present a protocol to isolate primary adult fibroblasts in an easy, fast and reliable way, performable by beginners (e.g., students). The procedure combines enzymatic tissue digestion and mechanical agitation with ultrasonic waves to obtain primary fibroblasts. The protocol can easily be adapted to specific experimental requirements (e.g., human tissue).

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