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High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

DOI :

10.3791/4007-v

8:48 min

June 28th, 2012

June 28th, 2012

11,223 Views

1Department of Entomology, Virginia Tech

Genome assemblies based on massively parallel DNA sequencing technologies are usually highly fragmented. The development of physical chromosome maps can potentially improve genome assemblies. Here, we demonstrate innovative approaches to chromosome preparation, fluorescent in situ hybridization, and imaging that significantly increase throughput of the physical map development.

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High throughput

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