Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
13.9K Views
•
12:42 min
•
November 26th, 2014
DOI :
November 26th, 2014
•Chapters in this video
0:05
Title
2:21
Preparing the FISH Oligonucleotide Probes
4:05
Preparing Slides for Immuno-FISH Using a Cytospin
6:23
Performing Immunoflourescence
8:36
Performing FISH
10:34
Results: Immuno–FISH Staining of Xist RNA and Histone H3K27me3
11:55
Conclusion
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