Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci
2.8K Views
•
09:07 min
•
March 4th, 2021
DOI :
March 4th, 2021
•1Laboratory of Nuclear and Genomic Health, Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Brunel University London, 2Biosciences, Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire
Chapters in this video
0:04
Introduction
0:46
Slide Preparation and Cell Culture
2:08
DNA Halo Preparation
3:22
Two‐Dimensional Fluorescence In Situ Hybridization
6:47
Results: DNA Halos for High‐Resolution Analysis of Genomic Interactions with the Nucleoskeleton
8:41
Conclusion
Related Videos
11:25
3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
10.2K Views
06:25
Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay
18.8K Views
17:14
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
13.9K Views
13:55
Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
18.3K Views
12:16
Robust 3D DNA FISH Using Directly Labeled Probes
34.5K Views
15:54
Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
27.7K Views
11:36
Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
18.0K Views
10:01
Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans
10.3K Views
08:52
Chemical Dimerization-Induced Protein Condensates on Telomeres
3.0K Views
08:31
Cytogenetics
42.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved