JoVE Logo
Faculty Resource Center

Sign In

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

DOI :

10.3791/4400-v

December 10th, 2012

December 10th, 2012

13,769 Views

1Department of Biochemistry and Molecular Biology, Knight Cancer Institute, Oregon Health & Science University

A quantitative method for the analysis of chromosome replication timing is described. The method utilizes BrdU incorporation in combination with fluorescent in situ hybridization (FISH) to assess replication timing of mammalian chromosomes. This technique allows for the direct comparison of rearranged and un-rearranged chromosomes within the same cell.

Tags

Chromosome Replicating Timing

-- Views

Related Videos

article

Double Whole Mount in situ Hybridization of Early Chick Embryos

article

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

article

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

article

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

article

In Situ Hybridization for the Precise Localization of Transcripts in Plants

article

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

article

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

article

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

article

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

article

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved