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Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans

DOI :

10.3791/4088-v

July 20th, 2012

July 20th, 2012

11,888 Views

1Department of Biochemistry, University of Washington, 2Molecular and Cellular Biology Program, University of Washington

This paper details how to use continuous-flow hypoxia chambers to generate atmospheres with defined concentrations of O2 to understand biological responses to decreased O2. This system is easy to setup and maintain, and flexible enough to suit a wide range of O2 concentrations and model systems

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Defined Gaseous Environments

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