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Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

DOI :

10.3791/4319-v

December 3rd, 2012

December 3rd, 2012

11,396 Views

1Department of Biological Sciences, University of North Texas

Described here is an in vivo technique to image sub-cellular structures in animals exposed to anoxia using a gas flow through microincubation chamber in conjunction with a spinning disc confocal microscope. This method is straightforward and flexible enough to suit a variety of experimental parameters and model systems.

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