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High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

DOI :

10.3791/4247-v

9:00 min

October 31st, 2012

October 31st, 2012

15,408 Views

1Pediatric Blood and Marrow Transplant Program, University of Michigan

High throughput validation of multiple candidate biomarkers can be performed by sequential ELISA in order to minimize freeze/thaw cycles and use of precious plasma samples. Here, we demonstrate how to sequentially perform ELISAs for six different validated plasma biomarkers1-3 of graft-versus-host disease (GVHD)4 on the same plasma sample.

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High Throughput Sequential ELISA

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