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A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

DOI :

10.3791/4368-v

May 1st, 2013

May 1st, 2013

12,918 Views

1Department of Experimental Oncology, European Institute of Oncology, 2Department of Pathology and Laboratory Medicine, European Institute of Oncology, 3U.O. Gastroenterologia 2, IRCCS Ca' Granda, Ospedale Policlinico di Milano

We introduce a novel method for the maintenance of human intestinal mucosa in culture and monitoring of the response to various types of stimuli over at least 24 hrs. With our method, the polarity of the tissue is maintained, allowing for a physiological stimulation via the apical route.

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