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Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

DOI :

10.3791/52483-v

6:33 min

March 6th, 2015

March 6th, 2015

33,674 Views

1Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, 2Department of Medicine, Section of Gastroenterology and Hepatology, Baylor College of Medicine

We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.

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Human Epithelial Enteroids

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