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Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

15.4K Views

09:16 min

April 20th, 2017

DOI :

10.3791/55687-v

April 20th, 2017


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Keywords Enteroendocrine Cells

Chapters in this video

0:05

Title

0:54

Tissue Preparation

3:15

Removal of Cell Debris and Single Cells

4:44

Collection of Crypt Fragments

6:55

Intestinal Culture Plating

7:38

Results: Secretion and Imaging Using Primary Small Intestinal Cultures

8:36

Conclusion

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