JoVE Logo
Faculty Resource Center

Sign In

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation

DOI :

10.3791/4454-v

May 2nd, 2013

May 2nd, 2013

15,855 Views

1Department of Surgery, University of Wisconsin-Madison, School of Medicine and Public Health

We describe a valuable diagnostic assay that could potentially be used to decide the withdrawal of immunosuppression after transplant without elevated risk of graft rejection. The assay uses the principles of Delayed Type Hypersensitivity and provides accurate assessment of both donor specific effector and regulatory immune responses mounted by recipients.

Tags

Trans vivo

-- Views

Related Videos

article

Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells

article

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

article

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

article

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

article

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells

article

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

article

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

article

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

article

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

article

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved