January 11th, 2013
•Visualizing individual cells in densely packed tissues, such as terminal Schwann cells (SCs) at neuromuscular junctions (NMJs), is challenging. "Sequential photo-bleaching" allows delineating single terminal SCs, for instance in the triangularis sterni muscle explant, a convenient nerve-muscle preparation, where sequential bleaching can be combined with time-lapse imaging and post-hoc immunostainings.
Tags
Related Videos
Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction
Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis
Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
Why Quantification Matters: Characterization of Phenotypes at the Drosophila Larval Neuromuscular Junction
In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
Measuring Neuromuscular Junction Functionality
Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction
In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved