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Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices

DOI :

10.3791/50034-v

October 29th, 2012

October 29th, 2012

18,545 Views

1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University

In this paper, we describe a useful method to study ligand-gated ion channel function in neurons of acutely isolated brain slices. This method involves the use of a drug-filled micropipette for local application of drugs to neurons recorded using standard patch clamp techniques.

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