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Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

DOI :

10.3791/50038-v

March 31st, 2013

March 31st, 2013

14,617 Views

1Department of Cell and Developmental Biology, State University of New York, Upstate Medical University, 2Department of Neurobiology and Anatomy, Eccles Institute of Human Genetics, University of Utah

Cilia-generated fluid flow in Kupffer’s Vesicle (KV) controls left-right patterning of the zebrafish embryo. Here, we describe a technique to modulate gene function specifically in KV cells. In addition, we show how to deliver fluorescent beads into KV to visualize fluid flow.

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