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Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics

DOI :

10.3791/59484-v

May 17th, 2019

May 17th, 2019

7,988 Views

1Department of Nutritional Sciences, University of Arizona, 2Cancer Biology Graduate Interdisciplinary Program, University of Arizona, 3Department of Chemical Engineering, University of Arizona

Using three-dimensional organotypic cultures to visualize morphology and functional markers of salivary glands may provide novel insights into the mechanisms of tissue damage following radiation. Described here is a protocol to section, culture, irradiate, stain, and image 50–90 μm thick salivary gland sections prior to and following exposure to ionizing radiation.

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Radiation Treatment

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