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Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

DOI :

10.3791/50064-v

May 1st, 2013

May 1st, 2013

14,113 Views

1Department of Pathology, University of Utah School of Medicine

We describe a robust method for chromatin immunoprecipitation using primary T cells. The method is founded on standard approaches, but uses a specific set of conditions and reagents that improve efficiency for limited a quantities of cells. Importantly, a detailed description of the data analysis phase is presented.

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