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Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development (Video) | JoVE

DOI :

10.3791/61516-v

8:07 min

July 1st, 2020

July 1st, 2020

6,949 Views

1Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", 2IRCCS Santa Lucia Foundation, 3Department of Biology and Biotechnology, "C. Darwin" Sapienza University, CNR - IBPM, 4CNR – Institute of Biomedical Technologies (ITB)

Here, we propose a method to efficiently obtain single muscle fibers at early post-natal developmental stages from homozygous mutant Lamin Δ8-11 mouse model, a very severe model for Emery-Dreifuss muscular dystrophy (EDMD).

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Single Myofiber Isolation

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