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Protein Transfection of Mouse Lung

DOI :

10.3791/50080-v

May 15th, 2013

May 15th, 2013

9,061 Views

1Department of Medicine, St. Luke's Roosevelt Medical Center

Transgenic mice or viral vectors have been used to increase protein expression within the lung. However, these techniques are time-consuming, technically challenging and have off-target effects that can confound results. Our protein transfection protocol uses a lipid based transfection reagent and an ultrafine microsprayer to uniformly deliver active protein to lung cells.

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