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Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy

DOI :

10.3791/50085-v

August 1st, 2013

August 1st, 2013

17,961 Views

1Cancer Institute, NYU Langone Medical Center, 2Infectious Diseases, NYU Langone Medical Center

The most commonly used method for generating large numbers of autologous dendritic cells (DCs) for use in tumor immunotherapy is described. The method uses IL-4 and GM-CSF to differentiate DCs from monocytes. The immature DCs are stimulated to mature and then loaded with antigens before they are injected back into the patient.

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Tumor Antigen loaded Mature Dendritic Cells

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