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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

DOI :

10.3791/50141-v

March 6th, 2013

March 6th, 2013

13,907 Views

1Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, 2The University of Melbourne

Biophysical and biochemical studies of interactions among membrane-embedded protein domains face many technical challenges, the first of which is obtaining appropriate study material. This article describes a protocol for producing and purifying disulfide-stabilized transmembrane peptide complexes that are suitable for structural analysis by solution nuclear magnetic resonance (NMR) and other analytical applications.

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