Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

0:34

Harvesting Cells After Blocking Free Cysteine Residues Using Iodoacetamide

2:02

Extraction of Protein and Sample Preparation

3:13

In Vivo Formaldehyde Cross-linking of Endogenous Proteins in U-2 OS Cells

4:30

Fractionation of Nuclei

6:11

Extraction of Protein and Sample Preparation

6:51

SDS-PAGE Analysis

7:53

Results: Analyzing Multimeric Complexes Using a Two-step Method of Non-reducing SDS-PAGE Analysis and Formaldehyde Cross-linking

9:04

Conclusion