JoVE Logo
Faculty Resource Center

Sign In

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

DOI :

10.3791/50167-v

March 17th, 2013

March 17th, 2013

31,867 Views

1Division of Infectious Diseases, New York State Department of Health

There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.

-- Views

Related Videos

article

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

article

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

article

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

article

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

article

T Cells Capture Bacteria by Transinfection from Dendritic Cells

article

In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System

article

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

article

Isolation and Activation of Murine Lymphocytes

article

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

article

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved