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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

DOI :

10.3791/50167-v

March 17th, 2013

March 17th, 2013

31,663 Views

1Division of Infectious Diseases, New York State Department of Health

There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.

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