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Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

DOI :

10.3791/55465-v

April 5th, 2017

April 5th, 2017

16,148 Views

1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH

We describe a method for generating Precision-cut Lung Slices (PCLS) and immunostaining them to visualize the localization of various immune cell types in the lung. Our protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.

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Keywords Precision cut Lung Slices

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