8.4K Views
•
08:48 min
•
October 25th, 2016
DOI :
October 25th, 2016
•Chapters in this video
0:05
Title
0:35
T Cell Receptor (TCR) Hemichain-encoded Retroviral Construct Preparation
1:41
Stable Packaging Cell Line Generation
3:08
Human T Cell Activation and Transduction
5:04
Novel Antigen-specific TCR Clone Reconstitution
6:25
Results: Centric TCR Hemichains Pair with Different Counter-chains to Form Antigen-specific TCRs with Varying Affinity
7:59
Conclusion
Related Videos
Retroviral Transduction of T-cell Receptors in Mouse T-cells
34.8K Views
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
8.6K Views
Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells
9.7K Views
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
13.2K Views
Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
9.5K Views
Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function
10.9K Views
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
8.6K Views
In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
9.8K Views
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
10.3K Views
Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
8.6K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved