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Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

DOI :

10.3791/54697-v

October 25th, 2016

October 25th, 2016

8,283 Views

1Department of Immunology, University of Toronto, 2Princess Margaret Cancer Centre, University Health Network

Herein we describe a novel method to generate antigen-specific T cell receptors (TCRs) by pairing the TCRα or TCRβ of an existing TCR, possessing the antigen-specificity of interest, with complementary hemichain of the peripheral T cell receptor repertoire. The de novo generated TCRs retain antigen-specificity with varying affinity.

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De Novo

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