January 31st, 2013
•Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.
Related Videos
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
Using Affordable LED Arrays for Photo-Stimulation of Neurons
Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
Optogenetic Stimulation of the Auditory Nerve
Optogenetic Functional MRI
Somatosensory Event-related Potentials from Orofacial Skin Stretch Stimulation
Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved