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Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

DOI :

10.3791/50184-v

January 31st, 2013

January 31st, 2013

14,458 Views

1Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles

Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.

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