Preparation of Light Emitting Diode (LED) for Blue Light Illumination
2:13
Imaging of Zebrafish Larvae Expressing Optogenetic TAR DNA-binding Protein 43 (TDP-43)
3:58
Light Stimulation of Optogenetic TDP-43h-Expressing Fish by Field Illumination of a Blue LED Light
4:35
Results: Characterization of TDP-43 Phase Transition in Spinal Motor Neurons of Zebrafish Larvae
6:34
Conclusion
Transcript
So this protocol enables us to induce pathological phase transitions of TDP-43 and address their cellular consequence in the spinal motor neurons in vivo, which is relevant for understanding why motor neurons degenerate in ALS. Due to the high tra
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We describe a protocol to induce phase transition of TAR DNA-binding protein 43 (TDP-43) by light in the spinal motor neurons using zebrafish as a model.