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Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

DOI :

10.3791/2561-v

March 31st, 2011

March 31st, 2011

27,661 Views

1Department of Cancer Biology, University of Massachusetts Medical School

A rapid, robust way of isolating viable adult epithelial stem cells from human skin is described. The method utilizes enzymatic digestion of skin collagen matrix , followed by plucking of hair follicles and isolation of single cell suspensions or tissue fragments for cell culture.

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Isolation

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