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Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

DOI :

10.3791/50193-v

March 7th, 2013

March 7th, 2013

14,978 Views

1Division of Hematology-oncology, Department of Medicine, Medical University of South Carolina

Determining donor cell engraftment presents a challenge in mouse bone marrow transplant models that lack well-defined phenotypical markers. We described a methodology to quantify male donor cell engraftment in female transplant recipient mice. This method can be used in all mouse strains for the study of HSC functions.

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