JoVE Logo
Faculty Resource Center

Sign In

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

DOI :

10.3791/50193-v

March 7th, 2013

March 7th, 2013

15,058 Views

1Division of Hematology-oncology, Department of Medicine, Medical University of South Carolina

Determining donor cell engraftment presents a challenge in mouse bone marrow transplant models that lack well-defined phenotypical markers. We described a methodology to quantify male donor cell engraftment in female transplant recipient mice. This method can be used in all mouse strains for the study of HSC functions.

-- Views

Related Videos

article

A Mouse Model of in Utero Transplantation

article

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

article

Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia

article

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

article

A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies

article

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

article

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

article

Ex Situ Normothermic Machine Perfusion of Donor Livers

article

Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR

article

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved