Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

10.5K Views

12:48 min

February 19th, 2013

DOI :

10.3791/50201-v

February 19th, 2013


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Xenopus Oocytes

Chapters in this video

0:05

Title

1:56

Pretreatment of Heterologous ATPase-expressing Oocytes

2:43

Electrophysiology

4:53

Rb+ Uptake Measurements

6:03

Preparation of AAnalyst 800

7:46

Results: Determination of Enzyme Kinetic Parameters

12:14

Conclusion

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