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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

DOI :

10.3791/51857-v

October 28th, 2014

October 28th, 2014

14,974 Views

1Institute for Physiology, Pathophysiology, & Toxicology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke, 2Institute for Immunology & Experimental Oncology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke

Calcium is involved in numerous physiological and pathophysiological signaling pathways. Live cell imaging requires specialized equipment and can be time consuming. A quick, simple method using a flow cytometer to determine relative changes in cytosolic calcium in adherent epithelial cells brought into suspension was optimized.

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Keywords Cytosolic Calcium

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