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Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

DOI :

10.3791/61401-v

8:33 min

March 11th, 2021

March 11th, 2021

1,498 Views

1Department of Physiology, Anatomy and Genetics, University of Oxford, 2Department of Chemistry and Neuroscience Program, Trinity College

This protocol presents a method for measuring adenine nucleotide binding to receptors in real time in a cellular environment. Binding is measured as Förster resonance energy transfer (FRET) between trinitrophenyl nucleotide derivatives and protein labeled with a non-canonical, fluorescent amino acid.

Tags

Nucleotide Binding

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