JoVE Logo
Faculty Resource Center

Sign In

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

DOI :

10.3791/61401-v

8:33 min

March 11th, 2021

March 11th, 2021

1,529 Views

1Department of Physiology, Anatomy and Genetics, University of Oxford, 2Department of Chemistry and Neuroscience Program, Trinity College

This protocol presents a method for measuring adenine nucleotide binding to receptors in real time in a cellular environment. Binding is measured as Förster resonance energy transfer (FRET) between trinitrophenyl nucleotide derivatives and protein labeled with a non-canonical, fluorescent amino acid.

Tags

Nucleotide Binding

-- Views

Related Videos

article

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

article

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

article

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

article

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

article

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

article

Author Spotlight: A Novel Method for Comprehensive Cell Component Analysis of Cerebral Blood Clots

article

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

article

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

article

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

article

Author Spotlight: Exploring Mitochondrial Function and Chemical Toxicity Using Drosophila melanogaster

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved