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Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

DOI :

10.3791/50248-v

April 12th, 2013

April 12th, 2013

10,526 Views

1Department of Craniofacial Biology, University of Colorado Denver Anschutz Medical Campus, 2Department of Cell and Developmental Biology, University of Colorado Denver Anschutz Medical Campus

A protocol for separation of embryo facial ectoderm and mesenchyme is described. We use Dispase II to treat whole embryos first, dissect whole facial prominences out, and then separate the facial ectoderm and mesenchyme.

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