JoVE Logo
Faculty Resource Center

Sign In

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

DOI :

10.3791/3854-v

9:38 min

June 21st, 2012

June 21st, 2012

89,379 Views

1Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics

The quality of mouse embryonic fibroblasts (MEFs) is dictated by the right strain of mouse such as CF-1. Pluripotency-supportive MEFs and conditioned media (CM) obtained from these should contain optimal concentrations of Activin A, Gremlin and Tgfβ1 needed for the Activin/Nodal and FGF pathways to co-operatively maintain self-renewal and pluripotency.

Tags

Mouse Embryonic Fibroblast Cells

-- Views

Related Videos

article

Propagation of Human Embryonic Stem (ES) Cells

article

Derivation of Human Embryonic Stem Cells by Immunosurgery

article

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

article

Culture and Maintenance of Human Embryonic Stem Cells

article

Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping

article

Freezing and Thawing Human Embryonic Stem Cells

article

Isolation and Derivation of Mouse Embryonic Germinal Cells

article

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

article

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

article

A System for ex vivo Culturing of Embryonic Pancreas

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved