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A System for ex vivo Culturing of Embryonic Pancreas

DOI :

10.3791/3979-v

August 27th, 2012

August 27th, 2012

13,367 Views

1Molecular and Cellular Basis of Embryonic Development, Max-Delbrück-Center for Molecular Medicine

Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.

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Ex Vivo Culturing

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