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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

DOI :

10.3791/1690-v

8:19 min

February 16th, 2010

February 16th, 2010

16,601 Views

1Department of Environment and Agro-Biotechnologies (EVA), Nutrition and Toxicology Unit (NuTox), Centre de Recherche Public-Gabriel Lippmann

Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.

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Toxin Induction

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