JoVE Logo
Faculty Resource Center

Sign In

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

DOI :

10.3791/4045-v

May 22nd, 2012

May 22nd, 2012

20,680 Views

1Faculty of Pharmaceutical Sciences, University of British Columbia

Tracking subtle changes in the progression and kinetics of cell cycle stages can be accomplished by use of a combination of metabolic labeling of nucleic acids with BrdU and total genomic DNA staining via Propidium Iodide. This method avoids the need of chemical synchronization of cycling cells, thereby preventing the introduction of non-specific DNA damage, which in turn affects cell cycle progression.

-- Views

Related Videos

article

Measuring Exocytosis in Neurons Using FM Labeling

article

Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

article

Flow Cytometry Purification of Mouse Meiotic Cells

article

Flow Cytometry-based Purification of S. cerevisiae Zygotes

article

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

article

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

article

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

article

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

article

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

article

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved