JoVE Logo
Faculty Resource Center

Sign In

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

DOI :

10.3791/50598-v

9:52 min

July 12th, 2013

July 12th, 2013

16,600 Views

1Department of Microbiology and Immunology, The Geisel School of Medicine at Dartmouth

Here we report a method for using type I and type II Δku80 strains of Toxoplasma gondii to efficiently generate targeted gene deletions and gene replacements for functional genomic analysis.

Tags

Genetic Manipulation

-- Views

Related Videos

article

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

article

Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions

article

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

article

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)

article

A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants

article

Forward Genetic Approaches in Chlamydia trachomatis

article

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

article

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

article

Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

article

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved