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A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants

DOI :

10.3791/3807-v

February 8th, 2012

February 8th, 2012

12,858 Views

1Department of Biology, Boston College

Forward genetics is a powerful method to unravel the molecular level of how Toxoplasma egresses from its host cell. Protocols are provided to chemically mutagenize parasites, enrich for mutants with defects in induced egress, and validate the phenotype of cloned mutants.

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Genetic Screen

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