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Forward Genetic Approaches in Chlamydia trachomatis

DOI :

10.3791/50636-v

October 23rd, 2013

October 23rd, 2013

12,609 Views

1Department of Molecular Genetics and Microbiology, Center for Microbial Pathogenesis, Duke University Medical Center

We describe a methodology to perform genetic analysis in Chlamydia based on chemical mutagenesis and whole genome sequencing. In addition, a system for DNA exchange within infected cells is described that can be used for genetic mapping. This method may be broadly applicable to microbial systems lacking transformation systems and molecular genetic tools.

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Genetic Analysis

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