Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

13.4K Views

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12:31 min

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May 1st, 2018

DOI :

10.3791/57357-v

May 1st, 2018

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Ganggang Huang*¹, Zhenwei Zhong*¹, Shane Miersch²^,³, Sachdev S. Sidhu¹^,²^,³, Shin-chen Hou¹, Donghui Wu¹

¹Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, ²Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, ³Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto

Transcript

Summary

Explore More Videos

Synthetic Phage Display

Fab Library

Antibody Library Construction

Antibody Diversity

Monoclonal Antibody Development

Phage Display Technology

Chapters in this video

0:04

Title

1:18

Electroporation and Calculation of the Library Size

6:02

Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing

9:43

Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size

11:07

Conclusion

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