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Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

DOI :

10.3791/62950-v

August 27th, 2021

August 27th, 2021

2,681 Views

1Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph

Ubiquitination is a critical protein post-translational modification, dysregulation of which has been implicated in numerous human diseases. This protocol details how phage display can be utilized to isolate novel ubiquitin variants that can bind and modulate the activity of E3 ligases that control the specificity, efficiency, and patterns of ubiquitination.

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