Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
1.9K Views
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06:33 min
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July 5th, 2021
DOI :
July 5th, 2021
•Cassandra Warden*1, Alan J. Simmons*2, Lejla Pasic3, Ashley Pitzer4,6, Sean S. Davies4, Justin H. Layer5, Raymond L. Mernaugh3, Annet Kirabo4,6
1Vanderbilt Eye Institute, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Department of Biochemistry, Vanderbilt University, 4Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, 5Division of Hematology and Oncology, Indiana University School of Medicine, 6Department of Molecular Physiology and Biophysics, Vanderbilt University
Chapters in this video
0:05
Introduction
0:54
Immunofluorescence
3:21
Negative Controls and Competitive Assays
4:37
Results: Detection of IsoLGs in Hypertensive and Normotensive Mice and Human Tissues
6:08
Conclusion
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