JoVE Logo
Faculty Resource Center

Sign In

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

DOI :

10.3791/50692-v

6:39 min

October 4th, 2013

October 4th, 2013

33,757 Views

1Vascular Biology Program, Department of Surgery, Boston Children's Hospital and Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering, Harvard University, 3Harvard School of Engineering and Applied Sciences

A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.

Tags

Intraductal Injection

-- Views

Related Videos

article

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation

article

The Microfluidic Probe: Operation and Use for Localized Surface Processing

article

Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats

article

Evaluation of Mammary Gland Development and Function in Mouse Models

article

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

article

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

article

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection

article

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

article

Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis

article

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved