JoVE Logo
Faculty Resource Center

Sign In

Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland

DOI :

10.3791/59502-v

June 7th, 2019

June 7th, 2019

12,061 Views

1Division of Genetics, Department of Medicine, Brigham and Women's Hospital, 2Department of Medicine, Harvard Medical School

The goal of this protocol is to describe a new breast cancer modeling approach based on the intraductal injection of Cre-expressing adenovirus into mouse mammary glands. This approach allows both cell-type- and organ-specific manipulation of oncogenic events in a temporally controlled manner.

-- Views

Related Videos

article

Intra-iliac Artery Injection for Efficient and Selective Modeling of Microscopic Bone Metastasis

article

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

article

Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis

article

Sectioning Mammary Gland Whole Mounts for Lesion Identification

article

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

article

Orthotopic Injection of Breast Cancer Cells into the Mice Mammary Fat Pad

article

Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad

article

Modeling Brain Metastases Through Intracranial Injection and Magnetic Resonance Imaging

article

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

article

Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved