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Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

12.0K Views

18:38 min

September 25th, 2013

DOI :

10.3791/50693-v

September 25th, 2013


Transcript

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Keywords RNA Interference

Chapters in this video

0:05

Title

2:54

How to Determine Plasmid Loss

4:32

Making Temporary Copies of the Library Omniplates

6:35

Preparation of Bacteria for Feeding Worms

9:28

Determining the Concentration of L1 Arrested C. elegans Larvae

10:53

dsRNA Feeding Screen

12:37

Results: Effects of egl-30, dpy-17, pat-10, and unc-4 Knockdown

17:50

Conclusion

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