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Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

DOI :

10.3791/50842-v

August 26th, 2013

August 26th, 2013

13,687 Views

1Department of Biophysical Chemistry, Institute for Physical Chemistry, University of Heidelberg, 2Max Planck Institute for Intelligent Systems at Stuttgart

We describe a method for accomplishing efficient immobilization of BMP-2 on surfaces. Our approach is based on the formation of a self-assembled monolayer to achieve the covalent binding of BMP-2 via its free amine residues. This method is a useful tool to study signaling at the cell membrane.

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