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Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

DOI :

10.3791/60934-v

10:37 min

March 16th, 2020

March 16th, 2020

8,935 Views

1Institute of Physical Chemistry, Albert-Ludwigs-Universität Freiburg, 2Chair in Polymer Chemistry, Saarland University, 3Cluster of Excellence livMatS at FIT - Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg

Covalent attachment of probe molecules to atomic force microscopy (AFM) cantilever tips is an essential technique for the investigation of their physical properties. This allows us to determine the stretching force, desorption force and length of polymers via AFM-based single molecule force spectroscopy with high reproducibility.

Tags

AFM based Force Spectroscopy

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