November 7th, 2013
•We developed an in vitro model that mimics an important component of the acute inflammatory response during infection of the bladder with uropathogenic Escherichia coli. The transuroepithelial neutrophil migration assay enables quantitative assessment of human neutrophil migration across bladder epithelia, cultured on permeable supports, in response to bacterial infection or chemoattractant substances.
Tags
Related Videos
Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay
Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
Human Neutrophil Flow Chamber Adhesion Assay
Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved