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Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

DOI :

10.3791/52687-v

April 16th, 2015

April 16th, 2015

28,047 Views

1LD MacLean Surgical Research Laboratories, Department of Surgery, McGill University

In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.

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